Study of Mussel adhesive protein (MAP) on adsorption solid surfaces by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0435-WXH)

Introduction

The blue mussel, Mytilus edulis, is known for its ability to adhere to hard surfaces in wave-swept seashores. This remarkable property of the mussel enables adherence to nearly all types of inorganic and organic surfaces in aqueous environments. An important group of these proteins are polyphenols called mussel adhesive proteins (MAPs) or Mytilus edulis foot proteins (Mefps). Successful attachment to Mefp-coated surfaces has been reported for various adherent cells, for example, neuronal cells, T47D human breast cancer cells, epiphyseal cartilage and osteosarcoma cells, and nonadherent cells such as T-lymphocytes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Study of the interaction between graphene oxide and lipid membranes
Development of adhesives relevant to scaffold fixation in orthopedic surgery.
Biosensors.
Biomaterial.
Immunological tests.
Drug-delivery schemes.

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis

Materials

• DPI biosensor
• DPI sensor chip

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