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Thermal diffusion describes the migration of molecules in a temperature gradient. The molecular origin of the effect, also called Ludwig-Soret effect, is one of the unsolved problems in physical chemistry. In some cases, even qualitative predictions are impossible. The main practical applications are separation processes such as thermal field flow fractionation of polymers and colloids or isotope separation, characterization of geochemical processes, and combustion. There are many experimental methods such as thermal diffusion cells, thermogravitational columns, thermal lens method, holographic grating methods, field-flow fractionation, and microscopic methods to investigate the thermal diffusion behavior of simple and complex liquid mixtures.