Study of the regulation of histone mRNA levels during the cell cycle by Fluorescence in situ hybridisation (FISH) (CAT#: STEM-MB-1224-WXH)

Introduction

Histone mRNA metabolism is tightly coupled to cell cycle progression and to rates of DNA synthesis. Each histone subtype has several copies of the same gene to allow for the large demand placed during DNA replication. Histones can be classified as replication dependent or replication independent, which is decided by their expression pattern during the cell cycle. The replication independent histone genes are transcribed at a relatively constant low rate, regardless of cell cycle stage. However, most of the vertebrate histone genes are replication dependent and are therefore more highly expressed during the cell cycle’s S phase.