Study of the surface membrane/T-tubular system and the sarcoplasmic reticulum in insect striated muscle stained with DilC18(3) by laser scanning confocal microscope technology (CAT#: STEM-MIT-0108-LJX)

Introduction

The myotube system is an important structure closely related to the contraction function of muscle fibers. It is composed of the sarcolemma that are recessed into muscle cells and sarcoplasmic reticulum . The sarcolemma is recessed into the muscle cells to form tubules, which run between myofibril in a direction perpendicular to myofibril, called transverse tube or T-tube.
The sarcoplasmic reticulum is a smooth endoplasmic reticulum, located between two transverse tubules. Longitudinally wrapped around each myofibril, called the longitudinal tubule; The two ends are enlarged into a flat sac, called the terminal cisterna. The sarcoplasmic reticulum stores calcium ions. When the sarcoplasmic reticulum receives excitation, calcium channels open, allowing a large amount of calcium ions stored in the sarcoplasmic reticulum to be released into the sarcoplasmic reticulum.

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Principle Applications Procedure Materials Notes Related Products

Principle

Laser scanning confocal microscope is a high-tech microscope. It is based on fluorescence microscope imaging and equipped with a laser scanning device, which uses ultraviolet or visible light to excite the fluorescence probe, thereby obtaining fluorescence images of the internal microstructure of cells or tissues.
The laser beam is used as the light source in the laser scanning confocal microscope. The laser beam passes through the illuminating pinhole and is reflected to the objective lens through the spectroscope. The laser beam is focused on the sample, and every point on the focal plane of the specimen is scanned. If there is a fluorescent substance that can be excited in the tissue sample, the fluorescence emitted after excitation is directly reversed back to the spectroscope through the original incident light path, and is first focused when passing through the detection pinhole. The focused light is detected and collected by the photomultiplier tube (PMT), and the signal is sent to the computer, and the image is displayed on the computer monitor after processing.

Applications

Imaging and analysis in the fields of morphology, molecular cell biology, neuroscience, pharmacology, genetics

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Striated muscle of honey bee

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures
In the starting sequence of the switch and in the scanning process, try to do fast and orderly, to protect the laser

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