Study on Binding of heparin to antithrombin III by Stopped-flow method (CAT#: STEM-AC-0026-WXH)

Introduction

Heparin is an anticoagulant (blood thinner) that prevents the formation of blood clots. Heparin is used to treat and prevent blood clots caused by certain medical conditions or medical procedures. It is also used before surgery to reduce the risk of blood clots.<br />Antithrombin III is a protein in the blood that blocks abnormal blood clots from forming. It helps the body keep a healthy balance between bleeding and clotting. Congenital antithrombin III deficiency is an inherited disease.<br />Antithrombin III activity is markedly potentiated by heparin, the principal mechanism by which both heparin and low–molecular-weight heparin result in anticoagulation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Stopped-flow is an experimental technique for studying chemical reactions with a half time of the order of 1 ms.
In its simplest form, a stopped-flow mixes two solutions. Small volumes of solutions are rapidly and continuously driven into a high-efficiency Ball mixer, so mixing is completed in just a few microseconds. This mixing process then initiates an extremely fast reaction.

Applications

Typically used to gain an understanding of reaction mechanisms, including drug-binding processes or following protein structural changes, stopped-flow spectroscopy enables the study of fast reactions in solution over timescales in the range of millisecond to hundreds of seconds.

Procedure

1. In stopped-flow experiments, two, three, or four sample solutions are rapidly mixed and injected into an observation cell.
2. When the flow is stopped, the kinetics are recorded with a detector best suited to the chemical properties of the solutions and the information of interest (e.g. particle size, the environment of the fluorophore, chromophore).

Materials

Stopped-Flows

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