Study on human hepatocellular carcinoma by spectral karyotyping (CAT#: STEM-ST-0374-WXH)

Introduction

The most common type of liver cancer starts in cells called hepatocytes and is called hepatocellular carcinoma. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.

Applications

Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.

Procedure

1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis

Materials

Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5

Other recommended products

Detection of unidentified chromosome abnormalities in human neuroblastoma by spectral karyotyping (SKY) (CAT#: STEM-ST-0384-WXH)
Spectral karyotyping of oocytes (CAT#: STEM-ST-0376-WXH)
Spectral Karyotyping Analysis of Head and Neck Squamous Cell Carcinoma (CAT#: STEM-ST-0379-WXH)
Karyotype analysis of human cytotrophoblasts (CAT#: STEM-ST-0385-WXH)
Detection of chromosomal aberrations by Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0373-WXH)
Spectral karyotype analysis of T-cell acute leukemia (CAT#: STEM-ST-0377-WXH)
Characterization of Chromosomal Rearrangements Using Spectral Karyotyping (CAT#: STEM-ST-0382-WXH)
Characterization of HeLa Cells by Spectral Karyotyping (CAT#: STEM-ST-0380-WXH)