Superresolution measurements in vivo: imaging Drosophila embryo by photoactivated localization microscopy (CAT#: STEM-MIT-0396-LJX)

Principles of photoactivated localization microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applied in many areas of the life sciences

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

• Sample Type:
Drosophila embryo

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures