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SWATH Technology for quantitative proteomics (CAT#: STEM-MB-0087-WXH)

Introduction

SWATH (Sequential Window Acquisition of all Theoretical fragment ions) technology is a DIA technology. It divides the entire mass spectrum scanning mass range into several small windows, and sequentially fragments all ions in each window, so that it can perform high-speed primary mass spectrometry scanning and secondary mass spectrometry analysis on all peptide ions in the scanning interval. Capable of quantifying nearly all detectable molecules in complex samples. DIA (data-independent acquisition) refers to the data-independent scanning mode, which is a holographic mass spectrometry data acquisition mode based on the electrostatic field orbitrap (Orbitrap).
Its advantages include high sensitivity, good repeatability, high quantitative accuracy, wide linear dynamic range, and high throughput.




Applications

• Drug target research
• Disease marker screening
• Plant stress/stress research
• Species protein sketch construction

Procedure

1. Protein purification, enzymatic hydrolysis and purification
2. Qualitative protein, determine parameters
3. Perform SWATH mass spectrometry analysis
4. Protein matching and quantitative information extraction
5. Data processing

Notes

Customers provide samples to be identified

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