T7 RNA Polymerase, 5000U, Seafrom (CAT#: STEM-CT-0092-ZJF)

The T7 RNA Polymerase, 5000U manufactured by Seafrom is suitable High-concentration T7 RNA polymerase for increased efficiency in in vitro transcription reactions and related research.
T7 RNA polymerase is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter sequence (5'-TAATACGACTCACTATAGGGAGA-3'). It can catalyze the incorporation of ribonucleotide triphosphates (NTPs) into a single-stranded or double-stranded DNA template downstream of the T7 promoter, thereby synthesizing RNA complementary to the DNA template downstream of the T7 promoter. Linear plasmids containing the T7 promoter or PCR products with the T7 promoter can be used as templates for in vitro RNA synthesis.

Source: Expressed in Escherichia coli, expressing the gene for bacteriophage T7 RNA polymerase
Specification: 5000U
Includes: 5×RNA Polymerase Reaction Buffer, Specification 3mL, 100 mM DTT, Specification 3mL
Storage Temperature & Shelf Life: Stored at -20°C, shelf life of 24 months
Enzyme Storage Buffer: 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 1 mM EDTA, 20 mM β-mercaptoethanol, 0.1% Triton® X-100, 50% glycerol
1×RNA Polymerase Reaction Buffer Composition: 40 mM Tris-HCl (pH 7.9), 10 mM DTT, 6 mM MgCl2, 2 mM spermidine
Enzyme Activity Unit Definition: In a 100μL system, 1 unit of enzyme is defined as the amount needed to incorporate 5 nmol of ATP into a polymeric nucleotide at 37°C within one hour
Enzyme Activity Test Conditions: In a 50μL reaction system containing 1×RNA Polymerase Reaction Buffer, 2mM NTPs (0.5mM each of ATP, UTP, CTP, GTP), 10mM DTT, and 1μg of T7 promoter-bearing DNA template
Quality Assurance: T7 RNA Polymerase does not contain other nucleases such as SP6 or T3 RNA polymerase, DNAases, and RNAases.

High Purity and Transcriptional Activity
Highly Specific Recognition of T7 Promoter
Nuclease and Protease-Free
Reduced Non-Specific Amplification
Stringent Quality Control
Manufactured in Gmp Compliant System

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