Th17 Pathway Assay (CAT#: STEM-MB-0355-WXH)

Introduction

Th17 cells are a subgroup of cells that secrete IL-17 (ie IL-17A) discovered in 2003, and together with Th1, Th2, and Tregs, they constitute four subgroups of CD4+ T cells. Th17 cells are differentiated from Th0 cells under the stimulation of IL-6 and TGF-β. It plays a key role in immune regulation, host defense and autoimmune diseases.
The effector molecules produced by Th17 cells mainly include IL-17A and IL-17F. IL-17A and IL-17F have similar functions and can act on a variety of cell types to induce the production of cytokines, chemokines and metalloproteinases. In addition, Th17 cells also secrete IL-21 and IL-22. IL-21 regulates the differentiation of Th17 in an autocrine form. IL-22 exerts its effects through its receptor.

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Principle Applications Procedure Materials Notes Related Products

Principle

Th17 cell differentiation mainly includes three stages: induction, expansion and stabilization. At each stage, the differentiation of Th17 cells is finely and complicatedly regulated by a variety of cytokines and signaling molecules.
In the induction phase, TGF-β and IL-6 are the main inducing factors to promote Th17 cell differentiation. Too high concentration of TGF-β will induce the expression of Foxp3 and antagonize the pro-differentiation effect of transcription factor RORγt, thereby inhibiting the differentiation of Th17 cells.
In the expansion stage, the cytokine IL-21 promotes or maintains the differentiation of Th17 cells through the autocrine method dependent on STAT3.
The stable phase is mainly maintained by IL-23. When IL-23 binds to its receptor, it can activate the JAK-STAT signaling pathway, which causes the phosphorylation of Jak2 and Tyk2, which in turn promotes the phosphorylation of STAT1, STAT3, STAT4 and STAT5.

Applications

To study the effect of each virus on Th17 cell differentiation signaling pathway
To study the regulation mechanism of Th17 cell differentiation signal pathway in disease
To study the effects of drugs or therapies on Th17 cell differentiation signaling pathways

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: IL-27, IL-2, IL-23, IL-6, TGFB, IL-15, STATS, STAT1, STATG, JUS, AHK, IKK, Tyk2, Tyk1, STAT3, mTCR, p52, IRF4, IRF6, HIF-1, IL-22, T-bet, IL-22, IL-21, GATA3, FOXP3, RXR, p38, SMADS

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