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The Determination of Chitosan with Some Anionic Surfactants by Resonance Rayleigh Scattering (CAT#: STEM-ST-0044-YJL)

Introduction

Chitosan, a typical polysaccharide comprising co-polymers of glucosamine and N-acetylglucosamine, is one of the most abundant natural polymers extracted from some fungi or from the shells of crustaceans such as lobsters, prawns and crabs. It can also be derived by the partial deacetylation of chitin. With both amino and hydroxyl groups, chitosan demonstrates unique exchange, chelation and adsorption abilities towards many metal cations and organic substances, which allows it to be widely used in analytical chemistry. Nowadays, chitosan has been proposed for many applications, such as water clarification, biomedical, pharmaceutical and cosmetic uses.
In recent studies some cationic surfactants, such as cetyldimethyl benzyl ammonium chloride, were utilized for the determination of nucleic acid and heparin with satisfactory results by the RRS technique, which shows that chemical reagents without chromophoric groups can also result in the enhancement of RRS signal.




Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system
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