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Chitosan, a typical polysaccharide comprising co-polymers of glucosamine and N-acetylglucosamine, is one of the most abundant natural polymers extracted from some fungi or from the shells of crustaceans such as lobsters, prawns and crabs. It can also be derived by the partial deacetylation of chitin. With both amino and hydroxyl groups, chitosan demonstrates unique exchange, chelation and adsorption abilities towards many metal cations and organic substances, which allows it to be widely used in analytical chemistry. Nowadays, chitosan has been proposed for many applications, such as water clarification, biomedical, pharmaceutical and cosmetic uses.
In recent studies some cationic surfactants, such as cetyldimethyl benzyl ammonium chloride, were utilized for the determination of nucleic acid and heparin with satisfactory results by the RRS technique, which shows that chemical reagents without chromophoric groups can also result in the enhancement of RRS signal.