The Determination of Sildenafil Citrate in a Pharmaceutical Formulation Using Evans Blue by Resonance Rayleigh Scattering Method (CAT#: STEM-ST-0025-YJL)

Introduction

Sildenafil citrate is a potent and selective inhibitor of cGMP (type V)-specific phosphodiesterase capable of enhancing relaxation of the penile corpus cavernosum, and therefore having the potential to improve penile erectile function. The activity of Sild is as an efficacious, orally active agent for the treatment of male erectile dysfunction, and is thus so currently being applied widely to clinical treatments. At present, most of the methods for the determination of Sild are based on HPLC, and electrochemistry. However, the procedures for many methods are not simple due to a preseparation required frequently before determining Sild; also, the sensitivity of some methods is not high enough.

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Principle Applications Procedure Materials Notes Related Products

Principle

Resonance Rayleigh scattering (RRS) is similar to Rayleigh scattering in nature. Resonance Rayleigh scattering is a special elastic scattering produced when the wavelength of Rayleigh scattering (RS) is located at or close to its molecular absorption band. The key to generating RRS is: when the scattering is at or close to the absorption band of the scattering molecule, since the electron absorbs the electromagnetic wave at the same frequency as the scattering frequency, the electron strongly absorbs the photon energy due to resonance and re-scatters. Its scattering intensity is several orders of magnitude higher than that of pure Rayleigh scattering, and it no longer obeys the Rayleigh law of I∝λ-4. This absorption-rescattering process is called resonance Rayleigh scattering (RRS).

Applications

Resonance Rayleigh scattering is used to the study of aggregation of chromophores on biological macromolecules and the determination of biological macromolecules such as nucleic acid, proteins and heparin, further, it has been used in the determination of trace amounts of inorganic ions and the cationic surfactant by means of ion association reactions with some dyes. In addition, it has been applied to the study of nanoparticles in liquid and the determination of β-cyclodextrin inclusion constant and the critical micelle concentration of surfactant.

Procedure

1. Sample preparation
2. Measurement by scattering detection instrument
3. Data analysis

Materials

Rayleigh scattering measurement system

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