Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM) (CAT#: STEM-MIT-0393-LJX)

Introduction

Actin is the structural protein of microfilaments and exists in two forms, monomer and polymer. A monomer actin, globular actin (G-actin), is a spherical molecule composed of a polypeptide chain. The actin polymers form actin filaments called fibros actin (F-actin).

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of photoactivated localization microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Applied in many areas of the life sciences

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
F-actin filaments

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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