Thrombocytes, eosinophile and basophile granulocytes in clinically healthy hens by the phase contrast microscope (CAT#: STEM-MIT-0261-LJX)

Introduction

Platelets are small pieces of cytoplasm shed by lysis of mature megakaryocytes in bone marrow. It's very important for the body to stop bleeding.
Eosinophils and basophils are both components of white blood cells and, like other granulocytes, are derived from blood-forming stem cells in the bone marrow. Eosinophils kill bacteria and parasites and are important cells in immune and allergic reactions.
Basophils are a type of white blood cell that originates from hematopoietic pluripotent stem cells in the bone marrow and enter the bloodstream after differentiation and maturation in the bone marrow. The increase of basophil number is more common in some allergic diseases, some blood diseases, some malignant tumors and some infectious diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

Phase contrast microscope is a microscope that uses the difference of refractive index and thickness between different structural components of an object to change the optical path difference through different parts of the object into the difference of amplitude (light intensity), and realizes observation through a condenser with a ring diaphragm and a phase objective.
Phase difference refers to the difference in phase caused by the same light passing through a medium with different refractive indices. Phase refers to the position reached by the fluctuation of light at a certain time. Generally, due to the small difference that the object being tested can produce, phase difference is difficult for the naked eye to distinguish, and it can only be distinguished after changing the difference to amplitude difference (brightness difference). The difference is determined by the difference in refractive index and thickness of the medium through which light waves pass, which is equal to the difference in refractive index multiplied by thickness (i.e. the difference in optical path). The phase contrast microscope uses the difference in optical path of the object being tested for microscopic examination.

Applications

For observation of living cells, non-stained tissue sections and the lack of contrast stained samples

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Thrombocytes, eosinophile and basophile granulocytes in clinically healthy hens

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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