Traceability of milk samples by isotope ratio mass spectrometry (CAT#: STEM-ST-0045-LJX)

Introduction

Cow milk samples from various provinces were collected, and the effects of lactation stage, sampling time, and geographic origin on the samples were studied by elemental analysis-isotope ratio mass spectrometry (EA-IRMS). Traceability accuracy was determined using δ13C, δ15N, δ2H and δ18O values to specifically assign geographic origin. Stable isotope ratios of C, N, H and O were not significantly different among three lactation stages; however the δ13C, δ15N, and δ18O values of milk were influenced by sampling time. Furthermore, there were highly significant regional differences in the mean δ13C and δ15N values of milk. In summary, the lactation stage had no effect on the traceability of milk, whereas sampling time and geographic origin did affect milk traceability. Different geographic locations with a separation distance greater than 0.7 km can be distinguished using multi-element (C, N, H, O) stable isotope ratio analysis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Isotope ratio mass spectrometry (IRMS) leverages magnetic sector mass spectrometry to enable high-precision measurement of the stable isotope content of a sample. Typical measurements target hydrogen, carbon, nitrogen, and oxygen analyses—although elements with masses up to and including sulfur can be measured. Solid, liquid, or gas phase samples are converted to simple gases then introduced to the IRMS. During analysis, an electron impact source ionizes sample-derived gas which is then accelerated down a flight tube, separated by mass, and quantified using a series of Faraday cups. The high precision of IRMS enables enumeration of even very small isotopic fractionation associated with physical, chemical, and biological transformations or natural abundance measurements.

Applications

For explaining the detailed molecular mechanisms behind biological processes
For understanding and quantifying nutrient and material exchanges between ecosystems
For providing ultra-precise stable isotope analyses
For understanding the geological history of the Earth
For food authenticity, forensic science, medical research and anti-doping testing

Procedure

1. Fill the reaction tube and install it, connect the gas path
2. Check for helium leaks
3. Heat up the reactor, wait for the reaction tube to burn stable, adjust the state of the equipment
4. Wrap the sample in a tin cup and test the sample
5. Store and process data

Materials

• Sample Type:
Milk

Notes

1.The approach is also valuable for quantifying the reactivity and progression of an applied stable isotope tracer to help determine reaction rates and final disposition of applied substrates.
2.IRMS offers a way of measuring isotopic variations with extremely high levels of accuracy. It can be used to detect isotope values of lighter elements with no issues, making it instrumental in the analysis of organic and natural samples.

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