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Transcriptome sequencing is the rapid and comprehensive acquisition of almost all transcripts and gene sequences of a specific cell or tissue of a species in a certain state through a next-generation sequencing platform. The advantage of sequencing technology is that the amount of data obtained by a single sequencing is large, higher coverage can be obtained, and more low-abundance transcripts can be detected, which is more advantageous in the transcriptome analysis of species with known genome sequences.