Transgenic Mouse Generation Service Based on CRISPR-Cas9 Technology

STEMart offers a cutting-edge Transgenic Mouse Generation Service, utilizing CRISPR-Cas9 technology to create customized transgenic mouse models. In our laboratory, this powerful gene-editing tool enables precise modifications at the DNA level, providing rapid and efficient results. With CRISPR-Cas9, we can achieve highly specific gene editing, leading to targeted mutations, knockouts, or insertions, facilitating various research projects across multiple disciplines.

CRISPR-Cas9 is a revolutionary tool that works by using a guide RNA to locate specific sequences within the genome, while the Cas9 enzyme makes precise cuts to modify the DNA. Its applications extend across genetics, molecular biology, developmental biology, and disease research, making it an invaluable asset for creating transgenic mouse models. The technique's simplicity, accuracy, and scalability are key factors that make it ideal for creating both simple and complex genetic alterations in mice.

Leveraging our expertise in CRISPR-Cas9 technology, we offer a comprehensive range of services, from initial design and targeting strategy development to mouse model creation and validation. Our services are tailored to meet the needs of researchers in diverse fields, including oncology, immunology, and neuroscience, ensuring precise, reliable, and timely results.

What We Offer

• Custom Gene Editing Service

Design and implementation of custom gene editing strategies based on CRISPR-Cas9 technology to create specific knockouts, insertions, or mutations in mice.

• Transgenic Mouse Model Creation Service

Comprehensive service that includes the generation, breeding, and validation of transgenic mouse models with specific genetic modifications.

• Knockout and Conditional Knockout Mouse Service

Targeted deletion of genes with CRISPR-Cas9 to create knockout or tissue-specific conditional knockout mouse models for advanced studies.

• Gene Repair and Replacement Service

Introducing specific gene repairs or replacements in mouse models to study disease mechanisms or therapeutic interventions.

Why Choose Us

• Precision and Efficiency

Our use of CRISPR-Cas9 allows for precise genetic modifications with high efficiency, reducing off-target effects and shortening project timelines.

• Comprehensive Support

From initial project consultation to post-editing validation, we provide full-spectrum support for your transgenic mouse projects.

• Customized Solutions

Our team tailors the gene-editing approach to meet the specific needs of your research, ensuring optimal results for various scientific inquiries.

Our Resources

CRISPR-Cas9 Gene Editing Platform

Our CRISPR-Cas9 system is powered by a Thermo Fisher Scientific platform, ensuring precision and flexibility for gene editing tasks across various mouse models.

Microinjection System (Eppendorf FemtoJet 4i)

We use the Eppendorf FemtoJet 4i for accurate delivery of gene-editing reagents into mouse embryos, enabling high-efficiency transgenesis.

Mouse Embryo Manipulation System (Leica DM6000B)

This high-performance microscope system allows for precise manipulation of mouse embryos during CRISPR-Cas9 experiments, ensuring accurate gene targeting.

STEMart provides high-quality animal model services tailored to meet the specific needs of our clients. Our team of experienced specialists utilizes cutting-edge equipment and adheres to rigorous quality control protocols, ensuring precise and dependable results. Contact us to learn more about our animal model services and how we can help you achieve your research goals.