Vascular Endothelial GF (VEGF) detection service (CAT#: STEM-MB-0253-WXH)

Introduction

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a highly specific vascular endothelial cell growth factor that promotes increased vascular permeability and extracellular Stromal degeneration, vascular endothelial cell migration, proliferation, and angiogenesis. Among them, VEGF-A plays an important role in the regeneration of blood vessels and endothelial cells. In addition, in the embryonic stage, the role of VEGF is to regulate the proliferation, metastasis of embryonic cells and improve the survival rate of endothelial cells, and to increase the efficiency of bone formation through the recruitment of osteoblasts and chondroblasts. Moreover, VEGF is involved in the onset and progression of many angiogenesis-dependent diseases, including cancer, certain inflammatory diseases, and diabetic retinopathy.

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Principle Applications Procedure Materials Notes Related Products

Principle

As a specific mitogen of vascular endothelial cells, VEGF can promote the growth of vascular endothelial cells in vitro and induce vascular proliferation in vivo. Especially in the hypoxic environment, VEGF binds to the VEGFR on the endothelial cell membrane, causing phosphorylation of the receptor, binding of the phosphorylated receptor to SHC, causing SHC phosphorylation, and activating SHC binding to the linker protein (GRB2) . There are two SH3 domains in Grb2, which can bind to guanine nucleotide release factor called SOS and change its activity. Then, SOS further activates Ras. Activated Ras acts on mitogen-activated protein kinase (MAPK) and activates ERK. Finally, ERK transfers into the nucleus, causing changes in the activity of certain transcription factors to achieve the mitogen properties of VEGF and induce endothelial cell proliferation.

Applications

Study of vascular endothelial cell migration, proliferation, and angiogenesis.
Study the formation of tumor blood vessels and the generation of tumor lymphatic vessels.
Study of the onset and progression of many angiogenesis-dependent diseases, including cancer, certain inflammatory diseases, and diabetic retinopathy.

Procedure

1. Process samples.
2. VEGF detection (Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Bone tissue, heart muscle, liver parenchyma, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipose tissue, etc.

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