Visualisation of liposomes prepared from skin and stratum corneum lipids by transmission electron microscopy technology (CAT#: STEM-MIT-0032-LJX)

Introduction

Liposome is an artificial membrane. In water, the hydrophilic head of phospholipid molecules was inserted into water, and the hydrophobic tail of liposome was extended to air. After agitation, the spherical liposome of double lipid molecules was formed, with diameter ranging from 25 nm to 1000nm.
Liposomes can be used in transgenic, or prepared, drugs, taking advantage of the fact that liposomes can fuse with cell membranes and deliver drugs into cells.
Transmission electron microscopy was used to visualize the liposomes prepared from total lipids extracted from mouse, human and porcine skin and stratum corneum.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Liposomes prepared from skin and stratum corneum lipids

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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