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Y-maze Test (CAT#: STEM-AE-0015-WXH)

Introduction

The Y-maze test is a behavioral test based on animals' natural exploratory curiosity, in which spontaneous alternating behavior is thought to reflect short-term spatial working memory. It takes advantage of the nature of the tested animals to explore new environments. During the experiment, the tested animals need to remember the previously explored directions every time they switch to explore new directions. Therefore, the Y-maze test can well reflect the spatial working ability of animals.
The Y-maze apparatus consists of 3 arms of equal length, divided into i novel arm, start arm and other arm. The angle between two adjacent arms is 120 degrees, and there is a movable partition in the center of each arm, and different geometric figures are pasted in each arm as a visual mark for the tested animal.




Applications

• It is used to evaluate the spatial working memory ability of rodents and the short-term memory ability of rats and mice.
• The main application areas are: functional decline, tropism, spatial working memory research, new drug development/screening/evaluation and other fields. Such as Alzheimer's disease animal model species, the evaluation of the spatial memory ability of the tested animals.

Procedure

1. Alternative behavior experiment in Y maze: put mice gently in the center of the maze, and allow them to explore freely for 8 minutes. The criteria for mice to enter each arm was full access of all four limbs. Sequential entry of mice into three different arms is an alternation. Compute alternate scores.
2. Electric stimulation Y maze experiment:
(1) Place a copper rod at the bottom of the maze device for electrical stimulation.
(2) The delay of each electric shock for each mouse is 5 s, and the response of escaping from the starting area to the safe area at one time is called "correct response", otherwise it is called "wrong response". 9 or more correct responses among 10 consecutive electric shocks are regarded as the society standard. Until the mouse has 9 consecutive correct responses during the training process, it is learned.
(3) The number of training times required for the tested animal to reach the learning standard after being shocked by the electric shock indicates the learning and memory performance, and the less the number of training times, the stronger the learning ability.

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