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Yeast surface display has been widely utilized to screen large libraries for proteins or protein fragments with specific binding properties. Yeast antibody display libraries can be coupled with FACS to select high-affinity antibodies that bind specifically to the targeted antigen by using labeled target antigens. Utilization of yeast antibody display combined with FACS allows a more controlled and quantitative selection process that can be fine-tuned to enrich clones with specific binding properties more effectively than phage display.