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Yeast Display Library Screening to study proeins with specific binding properties (CAT#: STEM-MB-0029-WXH)

Introduction

Yeast surface display has been widely utilized to screen large libraries for proteins or protein fragments with specific binding properties. Yeast antibody display libraries can be coupled with FACS to select high-affinity antibodies that bind specifically to the targeted antigen by using labeled target antigens. Utilization of yeast antibody display combined with FACS allows a more controlled and quantitative selection process that can be fine-tuned to enrich clones with specific binding properties more effectively than phage display.




Applications

• Antibody discovery
• Affinity maturation
• Humanization
• Identify enzyme inhibitor
• Map epitopes on antigens
• Discover/Validate new therapeutic targets
• Discover interaction between ligand and receptor

Procedure

1. Magnetic bead-based processes
2.First fluorescence-activated cell sorting (FACS):to ensure selection of yeast cell clones displaying full-length binders.
3.Second flow cytometry sorting: to enrich for higher affinity full-length binders to the target.
4.Individual clones of the population are identified by DNA sequencing and characterized using yeast surface titrations to determine the binding affinity (KD).
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