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ABI 7900 HT Fast Real-Time PCR System, Applied Biosystems (CAT#: STEM-PEPO-0046-ZJF)

Highlights

Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate.
Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.

Cat Number: STEM-PEPO-0046-ZJF

Application: For Real-Time PCR

Model: ABI 7900 HT Fast

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Description

The manufacturer of the ABI 7900 HT Fast Real-Time PCR System, Applied Biosystems is Applied Biosystems.
Refurbished Applied Biosystems (ABI) 7900HT Fast Sequence Detection System /Real Time PCR system ABI 7900HT Sequence Detection System software PC workstation. Optional: Bottom Reflective Plate, Plastic Lens Plate, Wood Lens Plate, TILDA Card Filling Station & well block kit (p/n 28000997).
The Applied Biosystems 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

Specification

Condition: Refurbished, Certified Pre-owned
Block Format: Interchangeable (96-Well or 384-Well)
Calibrated Dye: FAM™, JOE™, NED™, ROX™, SYBR® Green I, TAMRA™, TET™, VIC™
Capacity: Up to 36 x 96-well plates with optional automation accessory, Up to 84 x 384-well plates (with optional automation accessory and 384-well block)
Detection Method: Primer-Probe Detection, SYBR
Dimensions: 72 cm (W) x 84 cm (D) x 64 cm (H) (with drawer open)
Weight: 82kg
Dynamic Range: Up to 9 logs of linear dynamic range
For Use With (Equipment): 7900HT Fast System
Format: 96-well or 384-well plate
High Throughput Compatibility: High Throughput-Compatible, Multiplexing
Optics: Extended-life 488 nm argon-ion laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500 -650 nm)
Passive Reference Dye: No ROX, Other Passive Reference Dye, ROX (Pre-mixed), ROX (Separate Tube)
Reaction Speed: Fast
Reaction Volume Range: 10-30 µl for 96-Well / 5-20 µl for 384-Well
Run Time: 33 minutes (96-well in fast mode); <2 hrs (384-well in standard mode); 52 minutes (384-well in fast mode)
Sample Ramp Rate: Fast mode: ± 3.0°C/sec, Standard mode: ± 1.6°C/sec
Sensitivity: Down to 10 copies
Temperature Accuracy: ±0.25°C of display temperature
Temperature Range (Metric): 4-100°C
Temperature Uniformity: ±0.5°C
Thermal Cycling System: Peltier-Based System
Throughput: =10,000 endpoint PCR rxns per hour (384-well block and automation accessory), =5,000 real-time PCR rxns per day (384-well block and automation accessory)

Features

Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate.
Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.
96- or 384-well plate compatibility (including the TaqMan® Low Density Array).
Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security.
Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity.
Proven assay development guidelines save time and money

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