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Proteins are a very versatile group of macromolecules and they form the fundamentals of critical processes in the body. At the cellular level, they control cell division, metabolism and the movement of a variety of material and information into and out of cells. This ultimately has an impact on the structure, function and regulation of the tissues and organs in the body. Proteins differ from each other in their size, molecular structure and physiochemical properties. These differences allow for protein analysis and characterization by separation and identification. Protein analysis is important to understand the function of proteins and the impact of their presence, absence and alteration. This is key to advancing knowledge about diseases, providing the opportunity for biomarker discovery and development of therapeutics.