Knockout Mouse Generation Service Based on Embryonic Stem Cell Technology

STEMart offers a specialized Knockout Mouse Generation Service, utilizing embryonic stem cell (ESC) technology to create knockout mice for advanced research. In our laboratory, ESC-based gene targeting allows for precise disruption of specific genes, providing valuable models for studying gene function and disease mechanisms. This technology is essential for generating stable, inheritable knockout lines and is a powerful tool for long-term genetic research.

Embryonic stem cells possess the unique ability to differentiate into any cell type, making them ideal for genetic manipulation. The gene targeting process involves homologous recombination, where a designed DNA construct is integrated into the genome to disrupt a target gene. This method is widely used in fields like developmental biology, oncology, and immunology, where understanding gene function is critical for disease modeling and therapeutic discovery.

Leveraging our extensive expertise in ESC technology, we offer a comprehensive suite of services for generating knockout mouse models. From initial design and stem cell manipulation to mouse breeding and validation, our services cater to researchers in genetics, molecular biology, and beyond, ensuring high-quality, reproducible results.

What We Offer

• Custom Knockout Design Service

Tailored design and construction of targeting vectors to achieve precise gene knockout in mouse models using ESC technology.

• Homologous Recombination Service

Efficient gene targeting through homologous recombination in ESCs, ensuring the accurate disruption of specific genes.

• Germline Transmission Validation Service

Comprehensive validation to ensure that the genetic modification is successfully passed to offspring, confirming stable knockout lines.

• ESC Line Derivation Service

Creation and derivation of new ESC lines from different mouse strains, customized for your knockout experiments.

• Conditional Knockout Service

Generation of tissue-specific knockout mice through the combination of ESC technology and Cre-LoxP systems for controlled gene deletion.

Why Choose Us

• Proven Precision

Our ESC technology ensures precise and stable gene targeting, producing reliable knockout models with minimal off-target effects.

• Experienced Team

Our team of experts has extensive experience in embryonic stem cell manipulation, ensuring high success rates in gene targeting and knockout generation.

• Full Support from Design to Validation

We provide comprehensive support, including vector design, ESC manipulation, and full validation services, streamlining the knockout mouse production process.

• Custom Solutions

Our services are tailored to meet the unique needs of each research project, offering flexibility in vector design and ESC line derivation.

Our Resources

Gene Targeting Vector Design Platform

Our gene targeting vector design platform, powered by Thermo Fisher Scientific, allows precise construction of targeting vectors for ESC-based gene targeting.

Embryonic Stem Cell Culture System (MilliporeSigma MilliCell)

The MilliporeSigma MilliCell system supports the optimal growth and manipulation of ESCs, ensuring high efficiency in gene targeting procedures.

Microinjection System (Narishige IM-300)

This precise microinjection system facilitates the introduction of genetically modified ESCs into blastocysts, ensuring the accurate generation of knockout mice.

Mouse Breeding Facility (Tecniplast GM500)

Our state-of-the-art Tecniplast GM500 mouse breeding facility supports large-scale breeding and housing of genetically modified mice, ensuring stable and reproducible knockout lines.

STEMart provides high-quality animal model services tailored to meet the specific needs of our clients. Our team of experienced specialists utilizes cutting-edge equipment and adheres to rigorous quality control protocols, ensuring precise and dependable results. Contact us to learn more about our animal model services and how we can help you achieve your research goals.