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The research and development of new drugs is a long-term and complex process that requires strict scientific management and control. Among them, drug efficacy evaluation is one of the most important aspects, which can help scientific researchers judge whether a new drug is safe and effective and provide basic data and guidance for the clinical application of drugs. In vivo, pharmacodynamic evaluation is decisive for the identification of candidate molecules and the screening of clinical target indications and is an indispensable part of new drug development. A large variety of animal models has been developed to provide investigators with the ability to characterize the pharmacokinetics and pharmacodynamics of therapy.