Conditional Knockout Mouse Service Based on Cre-LoxP Technology

STEMart offers a specialized Conditional Knockout Mouse Service using Cre-LoxP technology to facilitate tissue-specific or time-specific gene deletion in mouse models. This technique is highly versatile, allowing for precise control over gene expression in specific cells or organs. In our laboratory, Cre-LoxP enables researchers to investigate gene functions that are critical for specific tissues or developmental stages, providing deeper insights into complex biological processes.

Cre-LoxP technology is based on the site-specific recombination system, where Cre recombinase recognizes and excises DNA sequences flanked by LoxP sites. This process allows for conditional gene deletion, activation, or inversion, depending on the design of the LoxP-flanked sequences. Cre-LoxP is widely used in developmental biology, cancer research, and neuroscience to study genes that may have lethal effects when globally knocked out, providing a powerful tool for controlled genetic research.

Leveraging our expertise in genetic engineering and the precision of Cre-LoxP technology, we offer a comprehensive range of services for creating conditional knockout mouse models. Our services are designed to meet the specific needs of researchers in fields like oncology, neurobiology, and immunology, ensuring accurate, reproducible results for advanced genetic studies.

What We Offer

• Tissue-Specific Gene Knockout Service

Using Cre-LoxP technology to delete genes in a specific tissue or cell type, allowing for targeted study of gene functions.

• Temporal Gene Knockout Service

Conditional knockout mouse models where gene deletion can be induced at a particular time during development or adulthood, providing temporal control of gene function.

• Gene Activation Service Based on Cre-LoxP System

Activation of genes at specific stages or in particular tissues using Cre-mediated recombination, enabling research on gene function in controlled conditions.

• Dual-Recombination System Service

Creating complex genetic models by combining Cre-LoxP with other recombination systems (e.g., FLP-FRT), enabling multi-level control over gene expression.

Why Choose Us

• Precision Control

Our Cre-LoxP system provides high precision in targeting specific tissues or developmental stages, ensuring accurate gene manipulation.

• Customizable Solutions

We tailor each project to your research needs, offering flexibility in gene targeting strategies and Cre-driver lines.

• Comprehensive Support

From vector design to validation, we offer full support throughout the entire conditional knockout generation process, ensuring high success rates.

Our Resources

Cre-LoxP Gene Editing Platform

Our Cre-LoxP platform is powered by Thermo Fisher Scientific, providing high accuracy in tissue-specific or time-specific gene recombination for conditional knockouts.

Embryo Manipulation System (Narishige MM-89)

This advanced embryo manipulation system allows precise microinjection of Cre constructs into mouse embryos, facilitating accurate gene targeting.

Fluorescent Imaging System (Zeiss Axio Observer 7)

Used to monitor Cre activity in live tissues, the Zeiss Axio Observer 7 allows for detailed observation of gene recombination in real-time, ensuring efficient knockout verification.

STEMart provides high-quality animal model services tailored to meet the specific needs of our clients. Our team of experienced specialists utilizes cutting-edge equipment and adheres to rigorous quality control protocols, ensuring precise and dependable results. Contact us to learn more about our animal model services and how we can help you achieve your research goals.