HOOK™ 6X His Protein Spin Purification Kit (Yeast), Cobalt Resin, Size 25, 786-633, G-BIOSCIENCES (CAT#: STEM-GT-3020-LGZ)

HOOK™ 6X His Protein Spin Purification Kit provides rapid purification of soluble 6X His-tagged proteins from yeast culture. Yeast is first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein while maintaining protein structure and activity. 0.5 ml of immobilized metal affinity resin was added to the clarified lysate, and the 6X His-tagged protein was purified by immobilized metal affinity chromatography (IMAC). The resin is transferred to a convenient spin column where it is washed quickly and the 6X His protein is eluted with imidazole buffer.

Yeast-pe LB™ is used to extract soluble proteins from yeast cells. Yeast PE LB™ is a patented improvement to Zymolyase®-based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffers utilizing mild non-ionic detergents and a proprietary combination of various salts and agents to enhance protein extraction and stability. A ready-to-use formulation of Zymolyase® is also provided. Depending on the application, other agents such as protease inhibitors can be added to Yeast PE LB™. This proprietary combination of reagents provides a simple and versatile method for yeast protein extraction. Yeast PE LB™ eliminates the need for glass bead lysis of yeast cells.

HOOK™ 6X His Protein Spin Purification Kit can be used for immobilized nickel chelate resin or cobalt chelate resin for metal affinity chromatography. Cobalt chelate resins have a lower binding affinity for 6X His tags compared to nickel chelate resins, which results in less non-specific binding and may result in slightly lower yields.

The HOOK™ 6X His Protein Spin Purification Kit is optimized to yield ~1 mg of soluble His-tagged protein with a purity of 80-90%, depending on the protein's expression level, resin type, conformation, and solubility characteristics.

For larger scale bacterial protein isolations, we recommend our gravity flow kit for 10mg protein extraction from yeast.

Size: 25

Isolate >1mg His tagged proteins from yeast
No sonication required, supplied with lysis buffer and enzyme
Nickel or Cobalt resins available
Spin column format

MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 50 K, 17-0291-50, Marvelgent biosciences (CAT#-STEM-GT-3055-LGZ)
MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 100 K, 17-0291-00, Marvelgent biosciences (CAT#-STEM-GT-3056-LGZ)
Capturem™ His-Tagged Purification Miniprep Kit, 20 Purifications , 635710, Takara Bio Inc (CAT#-STEM-GT-3057-LGZ)
Capturem™ His-Tagged Purification Maxiprep Kit, 6 Purifications , 635713, Takara Bio Inc (CAT#-STEM-GT-3058-LGZ)
Capturem™ His-Tagged Purification Large Volume, 4 Units, 635724, Takara Bio Inc (CAT#-STEM-GT-3059-LGZ)
Capturem™ His-Tagged Purification 24-Well Plate, 1 x 24-Well Plate, 635730, Takara Bio Inc (CAT#-STEM-GT-3060-LGZ)