HOOK™ 6X His Protein Spin Purification Kit (Bacteria), Cobalt Resin, Size 25, 786-629, G-BIOSCIENCES (CAT#: STEM-GT-3018-LGZ)

HOOK™ 6X His Protein Spin Purification Kit provides rapid purification of soluble 6X His-tagged bacterial culture proteins.

Bacteria are first lysed with bacterial PE LB™ and PE LB™-lysozyme, releasing total soluble protein while maintaining protein structure and activity. 0.5 ml of immobilized metal affinity resin was added to the clarified lysate, and the 6X His-tagged protein was purified by immobilized metal affinity chromatography (IMAC). The resin is transferred to a convenient spin column where it is washed quickly and the 6X His protein is eluted with imidazole buffer.

Bacteria - PELB™ Kit has been developed for the extraction of soluble proteins from bacterial cells. It is based on a patented improvement of lysozyme lysis, allowing extraction of soluble proteins while removing nucleic acids (DNA and RNA) released during cell lysis. Bacteria-pe LB™ Lysis eliminates viscosity build-up, allowing efficient clarification with lower centrifugal forces. Ultrasound examination is not required.

HOOK™ 6X His Protein Spin Purification Kit can be used for immobilized nickel chelate resin or cobalt chelate resin for metal affinity chromatography. Cobalt chelate resins have a lower binding affinity for 6X His tags compared to nickel chelate resins, which results in less non-specific binding and may result in slightly lower yields.

The HOOK™ 6X His Protein Spin Purification Kit is optimized to yield ~1mg/50ml of soluble His-tagged protein with a purity of 80-90%, depending on the protein's expression level, resin type, conformation, and solubility characteristics.

For larger scale bacterial protein isolations, we recommend our gravity flow kit to extract 10mg of protein from ~250ml of bacteria.

Size: 25

Isolate >1mg His tagged proteins from ~50ml bacterial culture
No sonication required, supplied with lysis buffer and enzyme
Nickel or Cobalt resins available
Spin column format

MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 10 K, 17-0291-10, Marvelgent biosciences (CAT#-STEM-GT-3053-LGZ)
MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 30 K, 17-0291-30, Marvelgent biosciences (CAT#-STEM-GT-3054-LGZ)
MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 50 K, 17-0291-50, Marvelgent biosciences (CAT#-STEM-GT-3055-LGZ)
MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 100 K, 17-0291-00, Marvelgent biosciences (CAT#-STEM-GT-3056-LGZ)
Capturem™ His-Tagged Purification Miniprep Kit, 20 Purifications , 635710, Takara Bio Inc (CAT#-STEM-GT-3057-LGZ)
Capturem™ His-Tagged Purification Maxiprep Kit, 6 Purifications , 635713, Takara Bio Inc (CAT#-STEM-GT-3058-LGZ)