HOOK™ GST Protein Purification Kit (Yeast), Size 5, 786-643, G-BIOSCIENCES (CAT#: STEM-GT-3010-LGZ)

HOOK™ GST Protein Purification Kit can purify soluble GST-tagged protein from yeast culture. Yeast is first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein while maintaining protein structure and activity. GST-tagged proteins were purified by passing the clarified lysate through prepacked columns.

Yeast-pe LB™ is used to extract soluble proteins from yeast cells. Yeast PE LB™ is a patented improvement to Zymolyase®-based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffers utilizing mild non-ionic detergents and a proprietary combination of various salts and agents to enhance protein extraction and stability. A ready-to-use formulation of Zymolyase® is also provided. Depending on the application, other agents such as protease inhibitors can be added to Yeast PE LB™. This proprietary combination of reagents provides a simple and versatile method for yeast protein extraction. Yeast PE LB™ eliminates the need for glass bead lysis of yeast cells.

The HOOK™ GST Protein Spin Purification Kit is optimized to yield up to 10 mg of soluble GST-tagged protein with a purity of 80-90%, depending on the protein's expression level, resin type, conformation, and solubility characteristics.

For small-scale, rapid isolation of GST proteins from yeast, we recommend our spin format kit.

Size: 5

Isolate >10mg GST tagged proteins from yeast
No sonication required, supplied with lysis buffer and enzyme

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