PRM (Parallel Reaction Monitor) is an ion monitoring technology based on high resolution and high precision mass spectrometry (such as Orbitrap series). Compared with the traditional SRM/MRM technology, PRM technology replaces the four-bar mass analyzer used to collect secondary information with a higher resolution and higher mass accuracy analyzer, and realizes the transformation from the detection of target ion pairs to the detection of all target ion fragments.
Applications
Amino acid analysis
Procedure
1. Protein extraction. 2. Protease hydrolysis, salt removal, freeze-drying. 3. Quantitative analysis, complete report collation.