CRISPR/Cas9 Gene Knock-in Mouse Model (CAT#: STEM-AE-0098-WXH)

Introduction

The sgRNA was designed and synthesized according to the gene sequence, and a homologous sequence containing a knock-in fragment or a point mutation was constructed for the target position, and co-injected with Cas9 mRNA into the cytoplasm of fertilized mouse oocytes. Cas9 nuclease, sgRNA, and genomic target sequence bind and cut double-stranded DNA, repair the genomic DNA with the homologous sequence containing the knock-in fragment as a template, and finally obtain a gene knock-in mouse that inserts a knock-in fragment or a point mutation in the target DNA sequence .