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Conditional Knockout Mice (CAT#: STEM-AE-0100-WXH)

Introduction

Conditional gene knockout mice: The deletion of the target gene occurs only in a certain stage of the mouse life cycle or in a specific tissue, while the expression is normal in other tissues or cells. Thus, the scope and timing of the modification of the mouse genome are in a controllable state.
Compared with conventional gene knockout, the advantage of conditional gene knockout is that it can prevent embryonic lethality and complete gene knockout at a specific time in a specific tissue.




Applications

• For the study of genes of interest with embryonic lethality.
• Used to study the physiological and pathological functions of genes in specific tissues.
• The expression of a gene can be regulated both in time and space.

Procedure

1. Determine the target gene and design the target.
2. Vector Construction.
3. Microinjection of Fertilized Eggs.
4. In vitro culture and transplantation.
5. Breeding identification.

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