Analysis of Proteins by Two-dimensional Electrophoresis (2-DE/2D-PAGE) (CAT#: STEM-ET-0344-ZJF)

Introduction

Two-dimensional gel electrophoresis (2-DE) is the benchmark method for the separation of intact proteins from complex mixtures. Many hundred different protein spots can be displayed from complex samples.
Based on 2-DE, we provide proteomic service including but not limited to the following:
• Protein cataloguing
• Protein quantification
• Analysis of protein species and isoform distribution
• Analysis of posttranslational modifications
• Protein and antibody quality control
• Host cell protein analysis
• Western blot
We accept samples including but not limited to the following:
• Purified proteins
• Frozen cell pellets or tissues
• Serum (with/without immunodepletion of abundant proteins) and plasma
• Body fluids (e.g. urine, cerebrospinal fluid (CSF), saliva, bronchoalveolar lavage)
• Tissues (e.g. myocardium, brain, kidney, liver, colon, artery tissue)
• Blood cells (e.g. monocytes, lymphocytes)
• Cell cultures (e.g. HeLa cells, human embryonic stem cells)
• Fermentation cultures (e.g. E. coli, bacillus caldolyticus, cyanobacteria)
• Microorganisms (e.g. trypanosoma brucei, bdellovibrio bacteriovorus, helicobacter pylorii, saccharomyces spp, penicillium spp)
• Plants (e.g. omphalotus olearius, arabidopsis thaliana, taxus baccata, potato tuber, tomato fruits)
• Other (e.g. insects, mollusca)
If you have any requirements or questions, please contact us for more details.