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Specific bacterial detection and identification is important in clinical diagnosis, food safety monitoring, and water quality control. Rapid and specific detection of bacteria is one of the earliest applications envisioned for FCM. Combined with immunofluorescent staining, FCM has been applied to specifically detect many pathogenic strains among other microorganisms, such as Haemophilus, Salmonella, Mycobacterium, Brucella, Branhamella catarrhalis, Mycoplasma fermentans, P.aeruginosa, Bacteroides fragilis, and Legionella.