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Morphological technique is one of the most commonly used neurobiological research techniques. Neural tissue consists of neurons and a variety of glial cells. There are hundreds of nuclear clusters in the brain, and the positions and functions of each cluster are very different. Therefore, morphological technique is of special significance to elucidate the structure and function of the nervous system. To study the histological structure of the central nervous system, the first step is to prepare histological sections. After the section is made, it needs to be dyed so that the tissue structure can be distinguished under the microscope. The prepared mouse tissue sections were dyed to observe the structure, so as to conduct morphological analysis.