Nanomanipulation of stereocilia of the cochlear hair cells for biophysical studies by atomic force microscopy (CAT#: STEM-SMMT-0008-LJX)

Introduction

Ear hair cells line the lymphatic fluid in the bone of the inner cochlea, which can respond to the mechanical wave conduction of the lymphatic fluid and generate excitement. Ear hair cells, which vary in length, are sensitive to mechanical waves of different wavelengths and are the receptors for human hearing.
Studies of the mechanoelectrical sensor system of the hair cell bundle in the cochlea require a manipulation device that enables controlled force application and movement of individual stereocilia in the nanometer range. Atomic force microscopy can do this.

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Principle Applications Procedure Materials Notes Related Products

Principle

Atomic force microscope (AFM) is a new type of surface analysis instrument based on the principles of physics and imaging through the interaction of scanning probe and sample surface atoms. It belongs to the third generation of microscopes after optical microscopes and electron microscopes.
AFM usually uses a sharp probe to scan the sample, which is fixed on a microcantilever that is extremely sensitive to the surface force between the probe and the sample. The deflection of the cantilever under force can cause the laser beam emitted by the laser source to shift after being reflected by the cantilever. The detector receives reflected light, and finally receives signals that are collected, processed, and formed into surface morphology images of the sample through a computer system.

Applications

Imaging of micro and nanoscale features on the surface of the sample
Suitable for research in fields such as materials science, biology, and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Cochlear hair cells

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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