Subcellular details of early events of differentiation induced by retinoic acid in human neuroblastoma cells detected by atomic force microscope (CAT#: STEM-SMMT-0018-LJX)

Introduction

Neuroblastoma cells are cancerous cells composed of immature nerve cells (neuroblasts). Neuroblastoma is the type of cancer that most commonly affects children. Atomic force microscopy (AFM) is gaining ever-increasing attention in biology as it allows us to achieve very high resolution both in air and in liquid. Recently, this technique has been employed for the observation of dynamic phenomena of cells in their culture medium. The service employed this technology for comparing different morphologies, neuronal and substrate-adherent type, of cell lines of human neuroblastoma, a tumor derived from neuroectodermal tissue.

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Principle Applications Procedure Materials Notes Related Products

Principle

Atomic force microscope (AFM) is a new type of surface analysis instrument based on the principles of physics and imaging through the interaction of scanning probe and sample surface atoms. It belongs to the third generation of microscopes after optical microscopes and electron microscopes.
AFM usually uses a sharp probe to scan the sample, which is fixed on a microcantilever that is extremely sensitive to the surface force between the probe and the sample. The deflection of the cantilever under force can cause the laser beam emitted by the laser source to shift after being reflected by the cantilever. The detector receives reflected light, and finally receives signals that are collected, processed, and formed into surface morphology images of the sample through a computer system.

Applications

Imaging of micro and nanoscale features on the surface of the sample
Suitable for research in fields such as materials science, biology, and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Human neuroblastoma cells

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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