Analysis Biomolecular Interactions of GR200 Aptamer with EGFR Protein by BLI (CAT#: STEM-MB-0168-CJ)

Introduction

EGFR is a transmembrane protein that is overexpressed in many types of cells with high proliferation activity, in particular, in aggressive cancers such as nonsmall-cell lung cancer and glioma. The binding of wild-type EGFR with epidermal growth factor (EGF) leads to autophosphorylation of the intracellular domain of EGFR and launches the phosphokinase cascade. Phosphokinases activate several transcription factors and provoke alterations in gene expression; cell proliferation is highly increased and upregulated. A large number of mutations within the EGFR gene have been annotated. One of the protein variants, EGFRvIII, has a deletion of 267 amino acids in the extracellular domain of EGFR; EGFRvIII is permanently activated without EGF binding, which results in high uncontrolled cell proliferation.