Analysis of CD48 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0855-LGZ)

Name: Analysis of CD48 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0855-LGZ
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Introduction

Official Full Name: CD48 molecule
Also known as: BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors, including the SLAM (signaling lymphocyte activating molecule) protein. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells, and endothelial cells, and is involved in the activation and differentiation pathways of these cells. However, the encoded protein has no transmembrane domain, but is anchored to the cell surface by GPI via a C-terminal domain that may be cleaved to generate a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.