Analysis of USH2A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0733-LGZ)

Introduction

Official Full Name: usherin
Also known as: US2; RP39; USH2; dJ1111A8.1
This gene encodes a protein containing a laminin EGF motif, a quinaxin domain, and numerous type III fibronectin motifs. The protein is found in the basement membrane and may play an important role in the development and homeostasis of the inner ear and retina. Mutations in this gene are associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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