Analysis of CTSD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2227-LGZ)

Introduction

Official Full Name: cathepsin D
Also known as: CPSD; CLN10; HEL-S-130P
This gene encodes a member of the peptidase A1 family. The encoded preproprotein undergoes proteolytic processing to generate a variety of protein products. These products include cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme has pepsin-like activity and plays a role in protein turnover and proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal lipoid browning-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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