Analysis of SPRR1B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0693-LGZ)

Introduction

Official Full Name: small proline rich protein 1B
Also known as: SPRR1; GADD33; SPR-IB; CORNIFIN
The protein encoded by this gene is an envelope protein of keratinocytes. The encoded protein is crosslinked to membrane proteins by transglutaminase, forming an insoluble layer under the plasma membrane. This protein is proline-rich and contains several tandem amino acid repeats.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ARHGEF16 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1260-LGZ)
Analysis of OR52E2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2340-LGZ)
Analysis of C1orf35 Gene (Mutation) in Hereditary Spastic Paraplegia by RT-qPCR (CAT#: STEM-MT-0202-LGZ)
Analysis of Mannheimia Ruminalis by Real-Time PCR Method (CAT#: STEM-MB-3706-LGZ)
Analysis of Salmonella Paratyphi B by Real-Time PCR Method (CAT#: STEM-MB-3412-LGZ)
Analysis of ITGA10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1090-LGZ)
Analysis of ASH1L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0786-LGZ)
Analysis of MIR4269 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1636-LGZ)