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Analysis of PSMA5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1216-LGZ)

Introduction

Official Full Name: proteasome 20S subunit alpha 5
Also known as: PSC5; ZETA
The proteasome is a multicatalytic protease complex with a highly ordered ring-shaped 20S core structure. The core structure consists of 4 rings of 28 different subunits; 2 rings of 7 α subunits and 2 rings of 7 β subunits. The proteasome is distributed in high concentrations in eukaryotic cells and cleaves peptides in a non-lysosomal pathway in an ATP/ubiquitin-dependent process. A modified proteasome, the essential function of the immunoproteasome is to process MHC class I peptides. This gene encodes a member of the T1A family of peptidases, the 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms of this gene have been found.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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