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HEK293T resDNA Quantitation Kit (qPCR), ACROBiosystems (CAT#: STEM-CT-0657-ZJF)

Highlights

High sensitivity for optimal product safety: LLOD (30 fg/µL) for detection of residue host cell DNA
High specificity: No cross-reactivity with unrelated DNA
Validation: ICH Q2(R2) as Validation of Analytical Procedures

Cat Number: STEM-CT-0657-ZJF

Application: resDNA Detection Kits

Model: OPA-R010

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Description

HEK293T resDNA Quantitation Kit is designed for quantitative detection of residual HEK293T DNA in biopharmaceutical productions (Antibodies, cells, viruses, etc.). Based on real-time quantitative PCR (qPCR) method, this kit makes detection the residual DNA form HEK293T cells rapid and reliable. The lower limit of quantitation is 30 fg/µL.All procedures are typically in less than 4 hours.
Use the kit after you extract host cell DNA from test samples. To achieve the best performances, resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) is commanded for DNA extraction.

Specification

Linearity: R²≥0.99, Eff%:90%-110%
Assay range: 300pg/uL-30fg/uL
LLOD: 15 fg/uL
Accuracy: 70%-130%
Repeatability: CV<20%
Time: 58 minutes
Assay tests: 100 tests
Storage: Store at-15~-30C for 12 months

Features

High sensitivity for optimal product safety: LLOD (30 fg/µL) for detection of residue host cell DNA
High specificity: No cross-reactivity with unrelated DNA
Validation: ICH Q2(R2) as Validation of Analytical Procedures
High-quality: This Kit is manufactured in Gmp facility and alignment with the ISO 13485 standard.

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