RNase Activity Assay Kit (Fluorescence), ACROBiosystems (CAT#: STEM-CT-0662-ZJF)

The RNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled RNA substrate. When the sample does not contain RNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains RNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em= 490/520 nm to determine whether the sample is contaminated by RNase.

Assay Type: FRET
Analyte: RNase A
Format: 96T/480T
Regulatory Status: RUO
Sensitivity: 0.03125 pg
Standard Curve Range: 0.03125 pg-2 pg
Assay Time: 30 min
Suitable Sample Type: For the quantitative determination of RNase A in the environment, some biological materials, common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage.
Sample volume: 10 μL & 80 μL

High recovery: Recovery of DNA fragments is up to 80%, superior yield and purity over columns.
High-quality: This Kit is manufactured in Gmp facility and alignment with the ISO 13485 standard.

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