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Molecular cloning is an essential process used in scientific research and discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then insert them into plasmids for replication and protein expression. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.