Recombinant Human B7-H3 Protein, Seafrom (CAT#: STEM-CT-0128-ZJF)

The Recombinant Human B7-H3 Protein manufactured by Seafrom is suitable for immune checkpoint regulation.
B7 homolog 3 (B7-H3), also known as CD276, is a member of the immunoglobulin superfamily. It contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 is thought to be involved in the regulation of T cell-mediated immune responses. It also plays a protective role in tumor cells by inhibiting cell lysis mediated by natural killer cells and serves as a marker for neuroblastoma cells. Additionally, B7-H3 is involved in the development of acute and chronic graft rejection reactions and the regulation of lymphocyte activity on mucosal surfaces. It also plays a crucial role in providing an appropriate immune environment for the placenta and the developing fetus throughout pregnancy.
Storage Information:
The lyophilized (freeze-dried) form can be stored at 4°C for 24 months, while the reconstituted liquid can be stored at -20°C for 6-12 months, with a recommendation to avoid repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: CD276, B7 homolog 3
Protein Sequence: The DNA sequence encodes human B7-H3 (UniProtKB/Swiss-Prot: Q5ZPR3.1), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human B7-H3 contains 433 amino acids, with a predicted theoretical molecular weight of 48.2 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Its ability to inhibit stimulation of human T cells induced by anti-CD3 was determined, with an ED50 of 5-15 μg/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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