Recombinant Rat IL-4 Protein, Seafrom (CAT#: STEM-CT-0058-ZJF)

The Recombinant Rat IL-4 Protein manufactured by Seafrom is suitable for studying anti-inflammatory effects and immune responses.
IL-4 is a pleiotropic cytokine that regulates various T cell and B cell responses, including cell proliferation, survival, and gene expression. It is produced by macrophages, T cells, and bone marrow stromal cells and plays a role in regulating the differentiation of naive CD4+ T cells into helper Th2 cells. Its cytokine secretion profile includes the production of IL-4, IL-5, IL-6, IL-10, and IL-13, which are favorable for humoral immune responses. Another major function of IL-4 is to regulate the switching of immunoglobulins to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells is associated with the production of IgE and allergies.
The lyophilized formulation can be stored at 2-8°C for 24 months. If further dilution is required, it can be reconstituted with injection-grade water, physiological saline, culture medium, or PBS. Once reconstituted, it should be aliquoted, and the aliquots should be stored at -20°C for 6 months or at -80°C for 12 months, with a post-dilution concentration not less than 100 μg/mL, while avoiding repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: BCGF, Bcf, BSF-1
Protein Sequence: DNA sequence encodes rat IL-4 (NP_958427.1) with a His tag at the C-terminus, contains 129 amino acids, predicted molecular weight of 14.6 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <1 EU/μg
Biological Activity: Depends on its ability to stimulate TF-1 cell proliferation, expected ED50 is 200-400 ng/mL
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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