Simultaneous assessment of radial and axial myocyte mechanics by combining atomic force microscopy and carbon fibre techniques (CAT#: STEM-SMMT-0015-LJX)

Introduction

Cardiomyocytes have transverse lines and are subject to vegetal innervation. It belongs to the involuntary muscle with transverse lines, which has the ability to contract excitingly.
Cardiomyocytes sense and shape their mechanical environment, contributing to its dynamics by their passive and active mechanical properties. While axial forces generated by contracting cardiomyocytes have been amply investigated, the corresponding radial mechanics remain poorly characterized. The service is to simultaneously monitor passive and active forces, both axially and radially, in cardiomyocytes freshly isolated from adult mouse ventricles.

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Principle Applications Procedure Materials Notes Related Products

Principle

Atomic force microscope (AFM) is a new type of surface analysis instrument based on the principles of physics and imaging through the interaction of scanning probe and sample surface atoms. It belongs to the third generation of microscopes after optical microscopes and electron microscopes.
AFM usually uses a sharp probe to scan the sample, which is fixed on a microcantilever that is extremely sensitive to the surface force between the probe and the sample. The deflection of the cantilever under force can cause the laser beam emitted by the laser source to shift after being reflected by the cantilever. The detector receives reflected light, and finally receives signals that are collected, processed, and formed into surface morphology images of the sample through a computer system.

Applications

Imaging of micro and nanoscale features on the surface of the sample
Suitable for research in fields such as materials science, biology, and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Adult mouse cardiomyocytes

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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