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High-resolution imaging of antibodies by tapping-mode atomic force microscopy (CAT#: STEM-SMMT-0023-LJX)

Introduction

Antibodies are proteins that are produced by the body as a result of the stimulation of antigens. It is a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. It is found only in the body fluids of vertebrates such as blood and on the surface of the cell membranes of their B cells.
Tapping-mode is an operation mode of atomic force microscope. Individual antibody molecules can be imaged using this mode.




Principle

Atomic force microscope (AFM) is a new type of surface analysis instrument based on the principles of physics and imaging through the interaction of scanning probe and sample surface atoms. It belongs to the third generation of microscopes after optical microscopes and electron microscopes.
AFM usually uses a sharp probe to scan the sample, which is fixed on a microcantilever that is extremely sensitive to the surface force between the probe and the sample. The deflection of the cantilever under force can cause the laser beam emitted by the laser source to shift after being reflected by the cantilever. The detector receives reflected light, and finally receives signals that are collected, processed, and formed into surface morphology images of the sample through a computer system.

Applications

Imaging of micro and nanoscale features on the surface of the sample
Suitable for research in fields such as materials science, biology, and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Antibodies

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures
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